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KMID : 1140120130180010088
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Cancer Prevention Research
2013 Volume.18 No. 1 p.88 ~ p.95
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Methanol Extract of Fructus liquidambaris Induces G1 Cell Cycle Arret in Human Colon Adenocarcinoma HT29 Cells
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Yun Hee-Jung
Jang Yeon-Kyeong
Choi Young-Hyun
Kim Byung-Woo
Kwon Hyun-Ju
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Abstract
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Fructus Liquidambaris, fructus of Liquidambar formosana Hance fructus, is traditional herbal medicine used in oriental region. There is no report for the anticancer activity of Fructus Liquidambaris yet. In this study, we investigated the anti-cancer effect of methanol extract of Fructus Liquidambaris (MEFL) in human colon adenocarcinoma HT29 cell. Treatment of MEFL to HT29 cells resulted in the growth inhibition and the G1 cell cycle arrest in a dose-dependent manner. In western blot analysis, the level of Cdk2, Cyclin D/E, phospho-pRb and E2F-1, which are G1-cell cycle-related proteins, were decreased in HT29 cells treated with MEFL. And the expression of phospho-p53 (Ser 15), phospho-Chk2 (Thr 68), p21 and p27 were increased, but Cdc25A was down-regulated in a dose-dependent manner. These results revealed that MEFL induces G1 cell cycle arrest through the induction of p21 and p27 by activated p53, and the reduction of Cdc25A by activated Chk2. This study is the first report about anticancer activity of MEFL, additional research is necessary
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KEYWORD
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Fructus liquidambaris, HT29 cells, G1 cell cycle arrest, p21, Cdc25A
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